Author: Aimee Neeley

HPLC Checklist V20191008

SeaBASS Submission Checklist: HPLC pigment data
Please fill out sections 1 and 2 below. Rename this file to be specific to your data, e.g., “checklist_hplc_MyCruiseName.rtf”

Experiment Name
______________________________________________________________________
Cruise Name
______________________________________________________________________

1) Sample collection method
	a. Filter type/pore size: 
	b. Vacuum pressure: 
c. Replicates collected (# or no): 
e. Flash frozen in L/N (y/n): 
f. Long term storage conditions/temperature: 

2) Sample measurement and analysis
a. Extraction 
1.	solvent: 
2.	volume added/delivery method: 
3.	disruption method, time: 
4.	soak time: 
5.	clarification method: 
b. HPLC analytical method
1.	Method reference: 
2.	Column: 
3.	Column temperature: 
4.	Mobile phase: 
5.	Flow rate: 
6.	Gradient: 
7.	Wavelengths monitored:	 
8.	Spectra collected? If so, what wavelengths:  
c. Internal standard 
1. Was an internal standard used? (If no, skip to ‘d’)
2. Name:
3. When it was added:
d. Instrument
1.	Make and model: 
2.	Injector type: 
3.	Injector precision (indicate if value is specific to data set or an average):
4.	Pump type: 
5.	Is sample compartment refrigerated? 
6.	Heated/thermostatted column compartment? 
7.	Detector type: 
	e. Instrument calibration
1.	Frequency: 
2.	Source of standards: 
3.	Single point or multi-point calibration: 
4.	How are calibration factors (or response factors. RF) calculated? 
5.	Is calibration accuracy monitored between calibrations? If so, how and how often?
6.	Noise at each wavelength:
f. Coelutions/Special Reporting
1.	Were there coeluting pigments (Rs <1.0)? Were the coeluting pigments reported separately or together? If reported separately, report resolution and computation method of separation.
2.	Critical pair (other than mentioned above), Rs: 
3.	Other notes on special reporting conditions?