Primary Production: Rates of primary production were measured utilizing standard 14C techniques as described in the JGOFS protocol (Knap et al. 1996)
Knapp, A.,A Michaels, A. Close, H Ducklow and A. Dickson (eds.) 1996. Protocols for the Joint Global Ocean Flux Study (JGOFS) Core Measurements. JGOFS Report Nr. 19, vi+170 pp. Reprint of the IOC Manuals and Guides No. 29, UNESCO 1994.
Chlorophyll: Chlorophyll a and phaeopigments were extracted in 90% acetone over 24h and determined according to Holm-Hansen et al. (1965) with a Turner AU-10 fluorometer calibrated with pure chlorophyll a (Sigma).
Holm-Hansen O, CJ Lorenzen, RW Holmes and JDH. Strickland. 1965. Fluorometric determination of chlorophyll.  Journal du Conseil.  Conseil International pour l'Exploration de la Mer, 30, 3-15.
Temperature: Temperature was determined by shipboard flow-through instrumentation and confirmed on discrete samples with thermometer.
Salinity: Discrete samples were measured using a refractometer. Underway measurements were made with shipboard flow-through instrumentation and confirmed with a Seabird CTD.
TOC: Samples were collected in acid washed 60ml bottles and analyzed according to the automated high temperature combustion technique outlined by Qian and Mopper (1996).
Qian JG, Mopper K (1996) Automated high-performance, hight temperature combustion total organic carbon analyzer. Anal. Chem. 68:2090-2097
Respiration: Bulk water respiration was measured using a modified Winkler method (JGOFS Protocols, Knap et al. 1996). 
Knapp, A.,A Michaels, A. Close, H Ducklow and A. Dickson (eds.) 1996. Protocols for the Joint Global Ocean Flux Study (JGOFS) Core Measurements. JGOFS Report Nr. 19, vi+170 pp. Reprint of the IOC Manuals and Guides No. 29, UNESCO 1994.
<3um: Cell counts were determined by flow cytometric analysis 
Sieracki ME, Poulton N, Crosbie N (2005) Automated Isolation Techniques for Microalgae, from Algal Culturing Techniques (edited Robert B Andersen) Elsevier Academic Press. 
0-20um: Cell counts were determined by flow cytometric analysis 
Sieracki ME, Poulton N, Crosbie N (2005) Automated Isolation Techniques for Microalgae, from Algal Culturing Techniques (edited Robert B Andersen) Elsevier Academic Press.
3-10um: Cell counts were determined by flow cytometric analysis 
Sieracki ME, Poulton N, Crosbie N (2005) Automated Isolation Techniques for Microalgae, from Algal Culturing Techniques (edited Robert B Andersen) Elsevier Academic Press.
Total Phytoplankton: Cell counts were determined by flow cytometric analysis 
Sieracki ME, Poulton N, Crosbie N (2005) Automated Isolation Techniques for Microalgae, in Algal Culturing Techniques (Robert B Andersen, Ed.) Elsevier Academic Press.
Eukaryotes: Cell counts were determined by flow cytometric analysis 
Sieracki ME, Poulton N, Crosbie N (2005) Automated Isolation Techniques for Microalgae, in Algal Culturing Techniques (Robert B Andersen, Ed.) Elsevier Academic Press.
Syn: Cell counts were determined by flow cytometric analysis 
Sieracki ME, Poulton N, Crosbie N (2005) Automated Isolation Techniques for Microalgae, in Algal Culturing Techniques (Robert B Andersen, Ed.) Elsevier Academic Press.
Bacteria: Cell counts were determined by flow cytometric analysis 
Sieracki ME, Poulton N, Crosbie N (2005) Automated Isolation Techniques for Microalgae, in Algal Culturing Techniques (Robert B Andersen, Ed.) Elsevier Academic Press.
Phosphate: Nutrient samples were frozen for later analysis following standard techniques 
Parsons TR, Maita Y, Lalli CM (1984) A manual of chemical and biological methods for seawater analysis, Vol. Pergamon Press, Oxford
Silicate: Nutrient samples were frozen for later analysis following standard techniques 
Parsons TR, Maita Y, Lalli CM (1984) A manual of chemical and biological methods for seawater analysis, Vol. Pergamon Press, Oxford
Nitrite+Nitrate: Nutrient samples were frozen for later analysis following standard techniques 
Parsons TR, Maita Y, Lalli CM (1984) A manual of chemical and biological methods for seawater analysis, Vol. Pergamon Press, Oxford
d13C/12C: Stable carbon isotope measurments were determined from POC samples. A CHN-IRMS was used to measure the samples.
Fry B, Sherr EB (1984) d13C measurements as indicators of carbon flow in marine and
freshwater ecosystems. Contributions to Marine Science 27:13-47.
PON: Samples were filtered onto a combusted GF/F filter and stored at -20C until analysis using high temperature combustion CHN analyzer at the University of California Santa Barbara.
Knapp, A.,A Michaels, A. Close, H Ducklow and A. Dickson (eds.) 1996. Protocols for the Joint Global Ocean Flux Study (JGOFS) Core Measurements. JGOFS Report Nr. 19, vi+170 pp. Reprint of the IOC Manuals and Guides No. 25, UNESCO 1994.
POC: Samples were filtered onto a combusted GF/F filter and stored at -20C until analysis using high temperature combustion CHN analyzer at the University of California Santa Barbara.
Knapp, A.,A Michaels, A. Close, H Ducklow and A. Dickson (eds.) 1996. Protocols for the Joint Global Ocean Flux Study (JGOFS) Core Measurements. JGOFS Report Nr. 19, vi+170 pp. Reprint of the IOC Manuals and Guides No. 25, UNESCO 1994.
C/N: These values were calculated based on the weight of carbon and nitrogen present in the POC and PON samples.