CHLa Method #1
MONOCHROMATIC; SPECTROPHOTOMETRIC DETERMINATION; CORRECTED FOR
PHEOPHYTIN AND TURBIDITY:  0.7 UM GFF FILTERS ARE PLACED IN 90% AQUEOUS
ACETONE AND GROUND TO A UNIFORM CONSISTENCY WITH A TISSUE GRINDER.
SAMPLES ARE STEEPED OVERNIGHT AT 4 DEGREES C IN THE DARK.  THE EXTRACT
IS CLARIFIED BY CENTRIFUGATION.  SPECTROPHOTOMETRIC OPTICAL DENSITY
READINGS ARE TAKEN BEFORE AND AFTER ACIDIFICATION USING 2 DROPS OF 1 N
HCL.  CHLOROPHYLL-A IS CALCULATED FROM OPTICAL DENSITY READINGS USING
LORENZEN'S  EQUATION:
CHLA=(26.7*((OD664B-OD750B)-(OD665A-OD750A))*EXVOL_ML)/(SAMVOL_L*LIPAT_C
M)				L01 	108	CHLA	ASTM 1979.
STANDARD PRACTICES FOR MEASUREMENT OF CHLOROPHYLL CONTENT OF ALGAE IN
SURFACE WATERS; METHOD D3731-79; PP. 1079-1083.	AMERICAN PUBLIC HEALTH
ASSOCIATION.  1985. STANDARD METHODS FOR EXAMINATION OF WATER AND
WASTEWATER; 16TH EDITION; METHOD 1002 G.
MONOCHROMATIC; SPECTROPHOTOMETRIC

CHLa Method #2
FILTERS ARE PLACED IN 90% AQUEOUS ACETONE AND GROUND TO A UNIFORM
CONSISTENCY WITH A TISSUE GRINDER.  SAMPLES ARE STEEPED OVERNIGHT AT 4
DEGREES C IN THE DARK.  THE EXTRACT IS CLARIFIED BY CENTRIFUGATION.  THE
FLUORESCENCE OF THE EXTRACT IS MEASURED BEFORE AND AFTER ACIDIFICATION
USING A FLUOROMETER CALIBRATED AGAINST A SPECTROPHOTOMETER USING PURE
CHL-A STANDARDS.			SEQUOIA TURNER FLUORMETER -
MODEL 112	L03 	69	CHLA	STRICKLAND AND PARSONS; 1972.  A
PRACTICAL HANDBOOK OF SEAWATER ANALYSIS.  BULLETIN 167 (2nd EDITION.)
FISHERIES RESEARCH BOARD OF CANADA.; OTTAWA; CANADA.	METHODS FOR THE
DETERMINATION OF CHEMICAL SUBSTANCES IN MARINE AND ESTUARINE MATRICES -
2ND Ed.; 1997.  METHOD 445.0			FLUOROMETRIC; IN-VITRO
CHLOROPHYLL A



TSS Method- A MEASURED SUBSAMPLE OF A WELL-MIXED WATER SAMPLE IS
FILTERED THROUGH A 0.7 um GLASS FIBER FILTER.  THE FILTER AND RESIDUE
ARE THEN DRIED AT 103 TO 105 DEGREES C.  WEIGHING IS DONE ON AN
ANALYTICAL BALANCE.  ALL FILTERS (CLEAN AND WITH RESIDUE) ARE STORED IN
A DESSICATOR PRIOR TO WEIGHING.  THIS PARAMETER IS ALSO CALLED
NON-FILTERABLE RESIDUE.	FROM DEC ALPHA	160.2		L01 	10
TSS	 APHA 1995. STANDARD METHODS FOR EXAMINATION OF WATER AND
WASTEWATER; 19TH EDITION; METHOD 2540 D. (16TH EDITION: METHOD 209 C;
PP. 96-97).	EPA. 1983. METHODS FOR CHEMICAL ANALYSIS OF WATER AND
WASTES.  USEPA-600/4-79-020. METHOD 160.2; PP. 160.2/1-3.
GRAVIMETRIC; DRIED AT 103-105 C