Checklist for SeaBASS submission: HPLC pigment data
Please fill out sections 1 and 2 below

Names of Experiment & Cruise: ___, ___

1) Sample collection method
	a. Filter type/pore size:
	b. Vacuum pressure:
    c. Replicates collected (# or no):   
    e. Flash frozen in L/N (y/n):
    f. Long term storage conditions/temperature:

2) Sample measurement and analysis
a. Extraction 
    1. solvent: 
    2. volume added/delivery method: 
    3. disruption method, time: 
    4. soak time: 
    5. clarification method: 

b. HPLC analytical method
    1. Method reference: 
    2. Column: 
    3. Column temperature:
    4. Mobile phase: 
    5. Flow rate: 
    6. Gradient: 
    7. Wavelengths monitored:	 
    8. Spectra collected? If so, what wavelengths:: 

c. Internal standard 
    1.Was an internal standard used? (If no, skip to ‘d’)
    2. Name:
    3. When it was added:

d. Instrument
    1. Make and model: 
    2. Injector type: 
    3. Pump type: 
    4. Is sample compartment refrigerated? 
    5. Heated/thermostatted column compartment? 
    6. Detector type: 

e. Instrument calibration:
    1. Frequency: 
    2. Source of standards: 
    3. Single point or multi-point calibration: 
    4. How are calibration factors (or response factors. RF) calculated? 
    5. Is calibration accuracy monitored between calibrations? If so, how and how often?
    6. Noise at each wavelength:

f. Coelutions/Special Reporting
    1. Were there coeluting pigments (Rs <1.0)? Were the coeluting pigments reported separately or together? If reported separately, report resolution and computation method of separation.
	2. Critical pair (other than mentioned above), Rs: 
    3. Other notes on special reporting conditions?

3) Data reporting should include
	a. Note size fractionated samples (separate fields, e.g., <pig>_#umprefilt)
	b. Instrument/injector precision (place in the header as comments?)
    c. Individual and summed pigments (based on SeaBASS fields/SeaHARRE reports)
	d. Mark ‘below detection limit’ values with up-to-date value (now = -8888)
	e. Report replicate filters separately