CHECKLIST FOR SeaBASS SUBMISSION: Extracted, fluorometric Chl V20220419 Please fill out the Collection, Measurement, and Analysis methods sections. Answer below each number. When finished, rename this file to be specific to your data, e.g. “checklist_extractedChl_EXPERIMENT_CRUISE_date_version.txt” Experiment Name: _______ Cruise Name: _______ ------------------------------- - SAMPLE COLLECTION METHODS - ------------------------------- 1) How were sampled collected, e.g., Niskin bottle on CTD rosette, or underway flow through seawater system? Also, if applicable, were the samples collected on upcast or downcast? 3) What was the vacuum pressure for filtration? 4) Indicate filter type/size used: 5) Samples size fractionated? (Y/N) If so, indicate filter types and pore sizes. 6) Were blank filters collected? (Y/N) 7) Were replicates collected? (Y/N) If so, how many? 8) Were the samples extracted fresh (soon after collection) or frozen (after storage)? 9) If not extracted fresh, how were the filters preserved immediately after collection? (e.g., Liquid nitrogen, dry ice) 10) (If applicable) How long were the filters stored before extraction? 11) What was the length time for extraction? -------------------------------- - SAMPLE MEASUREMENT METHODS - -------------------------------- 1) List the fluorometer make, model, excitation and emission settings (include lamp type and filter): 2) Describe method of fluorometer calibration and maintenance (include information on standards, number of calibration points, etc.): 3) What solvent was used for extraction (include %): 4) Describe measurement method (include literature reference; e.g., acidification method or Welschmeyer): 5) Note if blanks (solvent and/or filter) were measured: --------------------------------- - DATA ANALYSIS AND REPORTING - --------------------------------- Define equation(s) used to compute Chl and Pheo (if applicable). Include literature reference. **Report size-fractionated measurements as separate columns. **When reporting replicates, either report them separately or report a measure of uncertainty (e.g., standard deviation).