CHECKLIST FOR SeaBASS SUBMISSION: Particulate Filter Pad Absorption
V20201009
Please fill out the Collection, Measurement, and Analysis methods sections. Answer below each number. When finished, rename this file to be specific to your data, e.g. “checklist_particulate_absorption_EXPERIMENT_CRUISE_date_version.rtf”

Experiment Name: _______
Cruise Name: _______

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-  SAMPLE COLLECTION METHODS  -
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1) How were sampled collected, e.g., Niskin bottle on CTD rosette, or underway flow through seawater system? Also, if applicable, were the samples collected on upcast or downcast?

2) If applicable, was the bottle emptied into a large carboy for subsampling or was sampling done directly from bottle?

3) What was the vacuum pressure for filtration?

4) Were blank filters collected? (Y/N)

5) Were replicates collected? (Y/N) If so, how many?

6) Were the samples measured fresh (soon after collection) or frozen (after storage)?

7) In what type of container were the samples stored? (e.g., histoprep, foil, etc.)

8) How were the samples preserved immediately after collection? (e.g., Liquid nitrogen, dry ice)

9) What were the long-term storage conditions (e.g., temperature)?

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-  SAMPLE MEASUREMENT METHODS  -
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Note it is recommended that OD with blank filter subtracted should range between 0.1 and 0.4 per the IOCCG Absorption Coefficient Protocol

1) List the instrument make, model and accessories:

2) List instrument calibration, performance tests and maintenance performed:

3) What was the method of measurement? (e.g., transmittance, transmittance-reflectance, inside sphere, etc.)

4) List any references for your protocol:

5) Provide filter pad scan settings
a. Wavelength range:
b. slit band width:
c. scan speed:

6) How were the blank filters measured?

7) Were air scans measured to monitor instrument stability?

8) How many filter rotations were measured?

9) What was the extraction method? Include concentration. (e.g.,95% methanol, hypochlorite, etc.)

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-  DATA ANALYSIS METHODS  -
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1) Describe filter blank and air scan subtractions, where applicable

2) Define scatter correction/null correction method (if using transmittance method)

3) Define which beta amplification correction that was used, with citation

4) Were the sample absorbance scans smoothed (e.g. moving box average filter)? If so, describe the method used.