CHECKLIST FOR SeaBASS SUBMISSION: Particulate Filter Pad Absorption (V20241017)

Please fill out the Collection, Measurement, and Analysis methods sections. When finished, rename this file to be specific to your data, following this schema: checklist_particulate_absorption_EXPERIMENT_CRUISE_SubmissionDate_SeaBASSfileVersion.txt (e.g., checklist_particulate_absorption_EXPORTS_EXPORTSNA_20241016_R1.txt)

Experiment Name: 
Cruise Name: 
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-  SAMPLE COLLECTION METHODS  -
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1) How were sampled collected, e.g., Niskin bottle on CTD rosette, or underway flow through seawater system? If applicable, were the samples collected on upcast or downcast?

2) Was the bottle emptied into a large carboy for subsampling or was sampling done directly from bottle?

3) What was the vacuum pressure (mm Hg) for filtration?

4) Were blank filters collected? (Y/N)

5) Were replicates filters collected and analyzed? (Y/N) If so, how many? (Note that replicate filter data should be prepared in submitted in separate SeaBASS files)

6) Were the samples measured fresh (soon after collection) or frozen (after storage)?

7) In what type of container were the samples stored? (e.g., histoprep, foil, etc.)

8) Were samples flash frozen in liquid nitrogen immediately after collection? If not then please describe preservation method prior to long-term freezer storage.

9) At what temperature we samples stored in a freezer and were there any periods when samples were stored in an alternative method? (e.g., Sample were stored shipboard in Š80 freezer and transported in liquid nitrogen dewar, which maintained temperature during approximately 1 week of transit to laboratory Š80 freezer)

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-  SAMPLE MEASUREMENT METHODS  -
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Note it is recommended that OD with blank filter subtracted should range between 0.1 and 0.4 per the IOCCG Absorption Coefficient Protocol

1) List the instrumentÕs make, model, and any accessories:

2) List instrumentÕs calibration, performance tests, and date of latest maintenance:

3) What was the method of measurement? (e.g., transmittance, transmittance-reflectance, inside sphere, etc.)

4) Was an integrating sphere used (yes/no)?

5) List any references for your protocol:

6) Provide filter pad scan settings
a. Wavelength range:
b. slit band width:
c. scan speed:

7) How were the blank filters measured?

8) Were air scans measured to monitor instrument stability (yes/no)?

9) How many filter rotations were measured and at which angles? If multiple filter rotations were averaged together then please report standard deviation (_sd) or standard error (_se) AND the number of filter rotation scans (_bincount) 

10) What was the extraction method? Include concentration. (e.g., 95% methanol, hypochlorite, etc.)

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-  DATA ANALYSIS METHODS  -
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1) Describe if filter blank and air scan subtractions, where made and used, if applicable.

2) Define scatter correction/null correction method (if using transmittance method). The absorption coefficients may be null corrected; however, the raw absorbance values must NOT include null correction. Intermediate steps are not included in the submission. Null correction should be reported in the headers if used (e.g. /null_correction=abs_ap:0.001,abs_ad:0.005)

3) Define which beta amplification correction that was used, with citation.
4) If there are any additional calculations used to go from raw absorbance values to the data within your file please describe it here:

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-  REMINDERS/NOTES  -
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1) Do not apply smoothing algorithms to the optical densities or coefficients. 
2) If data was collected below 400nm, SeaBASS requires all NASA funded PIÕs to submit at least the optical densities (raw absorbances) below 400 nm.
3) During QA/QC, SeaBASS will recalculate the absorption coefficients, if the data do not match our results, the dataset will be returned to the PI for clarification and potential resubmission of corrected data.