Plankton and other Particles (IFCB, UVP)

The guidelines presented below will assist the user in submitting imaging-in-flow or submersible microscopic data to SeaBASS. New metadata headers and fieldnames have been developed for plankton and other particle data. Additionally, detailed instructions are given for submission of documentation (protocol document, checklist, etc.), images and additional ancillary files. The submitter must indicate in the checklist whether all ROIs in a sample were annotated. Greater detail regarding the development and application of these guidelines and requirements can be found in Neeley et al., In Press.

 
**Neeley, A., S. Beaulieu, C. Proctor, I. Cetinic, J. Futrelle, I. Soto Ramos, H. Sosik, E. Devred, L. Karp-Boss, M. Picheral, N. Poulton, C. Roesler, and A. Shepherd. (2021) Standards and practices for reporting plankton and other particle observations from images. 38 pp. DOI:10.1575/1912/27377 

Plankton and particles imagery (IFCB, UVP)

The guidelines presented below will assist the user in submitting imaging-in-flow or submersible microscopic data to SeaBASS. New metadata headers and field names have been developed for plankton and other particle data. Additionally, detailed instructions are given for the submission of documentation (protocol document, checklist, etc.), images, and additional ancillary files. The submitter must indicate in the checklist whether all ROIs in a sample were annotated. Greater detail regarding the development and application of these guidelines and requirements can be found in Neeley et al., 2021.

 
**Neeley, A., S. Beaulieu, C. Proctor, I. Cetinic, J. Futrelle, I. Soto Ramos, H. Sosik, E. Devred, L. Karp-Boss, M. Picheral, N. Poulton, C. Roesler, and A. Shepherd. (2021) Standards and practices for reporting plankton and other particle observations from images. 38 pp. DOI:10.1575/1912/27377 

Plankton and Particles Flow Cytometry

The guidelines presented below will assist the user in submitting flow cytometry data to SeaBASS. The format described herein allows for Level 2 submissions of flow cytometry data, meaning sums of total abundances of each phytoplankton or bacterial group. New metadata headers and field names have been developed for these data. Additionally, detailed instructions are given for the submission of documentation (protocol document, checklist, etc.), images, and additional ancillary files. Greater detail regarding the development and application of these guidelines and requirements can be found in Neeley et al., 2023.

 

Neeley, A.R., Soto-Ramos, I. and Proctor, C. (2023) Standards and Best Practices for Reporting Flow Cytometry Observations: a technical manual, Version 1.1. Greenbelt, MD., NASA Goddard Space Flight Center, 31pp. DOI: http://dx.doi.org/10.25607/OBP-1864.2.